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shuttle plasmid (pSET1 or pVA838) construct made up of the functional copy with the mutated gene fused in-body to your cloned S. suisreceiver cells throughout the 89K-encoded GI-form T4SS transportation conduit. So far, though mobilization of genetic factors by T4SSs continues to be well studied in Gram-unfavorable microbes, the transfer of Gram-go